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KMID : 0364819910290050278
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Korean Journal of Microbiology
1991 Volume.29 No. 5 p.278 ~ p.283
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Effect of Amino acid Substitutions of Escherichia coli r-Glutamylcysteine Synthetase
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Nam, Yong Suk/남용석
Kim, Joong Soo/Kwak, Joon Hyeok/Park, Young In/Lee, Se Yong/김중수/곽준혁/박영인/이세영
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Abstract
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1
Two amino acid residues (Ala´ and Ser´) of E coli y-glutamylcysteine synthetase have been investigated whether they are the site of feedback inhibition by site specific mutagenesis. Single substitution of serine"s (S495F), and double substitutions of alanine´ and serine"s (A494G-S495F) resulted in the inactivation of the y-glutamylcysteine synthetase activity. Substitution of alanine´ with glycine´ resulted in a higher level of feedback inhibition. These results suggest that serine°´S in y-glutamylcysteine synthetase is required for its catalytic activity and alanine´ is presumably related to the feeback inhibition site.
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